Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: ( A ) Representative sigmoid curve revealing single-digit nanomolar affinity of DA364 for the human α v β 3 integrin receptor. ( B ) Dose-response inhibition of DA364 uptake in human melanoma WM266 cells by the unconjugated cRGD vector.

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Inhibition, Plasmid Preparation

Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: The workflow consists of the intravenous administration of DA364 24 h or 48 h prior to surgery ( A ), followed by pre-operative imaging ( B , C : procedure and fluorescent image in panel respectively), intra-operative imaging ( D , E : procedure and correspondent fluorescent image in panel respectively) and post-operative imaging ( F , G : procedure and correspondent fluorescent image, visualized on screen, respectively). Excised tissues were sliced in 4 μm-thick sections and underwent tumor assessment by Hematoxylin and Eosin histopathology ( H ), and evaluation of the β 3 integrin by immunohistochemistry ( I ).

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Imaging, Histopathology, Immunohistochemistry

Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: ( A ) Western blot analysis of integrin receptor subunits expression per tumor type. Black circles, malignant tumors; grey circles, benign tumors. Horizonal bars represent medians. ( B ) Representative image of β 3 integrin immunostaining on a mammary gland adenocarcinoma showing tumor cell and stromal staining (brown, β 3 integrin positive stain; cell nuclei are stained with Hematoxylin in blue). Scale bar, 100 microns.

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Western Blot, Expressing, Immunostaining, Staining

Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: Semi-quantitative (score) and quantitative (area) evaluation of anti-b 3 integrin staining in tumor samples

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Staining

Journal: Biomedicines

Article Title: Triazole Modified Tetraiodothyroacetic Acid Conjugated to Polyethylene Glycol, a Thyrointegrin α v β 3 Antagonist as a Radio- and Chemo-Sensitizer in Pancreatic Cancer

doi: 10.3390/biomedicines10040795

Figure Lengend Snippet: Signaling pathways significantly affected by the P-bi-TAT treatment of human pancreatic carcinoma SUIT2 cells.

Article Snippet: Vibrant MTT cell proliferation assay kit was purchased from Life Technologies Corporation (Carlsbad, CA, USA), and fluorescein isothiocyanate (FITC) conjugated antibody of integrin α v β 3 was purchased from BD Biosciences (San Jose, CA, USA).

Techniques: Ligand Binding Assay, Activity Assay, Homologous Recombination

Journal: Molecular Biology of the Cell

Article Title: Osteopontin inhibits osteoblast responsiveness through the down-regulation of focal adhesion kinase mediated by the induction of low–molecular weight protein tyrosine phosphatase

doi: 10.1091/mbc.E16-10-0716

Figure Lengend Snippet: CD44 is involved in the induction of LMW-PTP expression by OPN. (A) MC3T3-E1 cells were treated with 10 μM cyclic RGD peptide (an integrin α V β 3 –specific antagonist), anti-CD29 neutralizing antibody, or anti-CD44 neutralizing antibody for 6 h, followed by OPN stimulation at 100 ng/ml for 3 h. Lmw-ptp mRNA induction was analyzed by real-time RT-PCR. The same experiments were performed at least three times with consistent results. Relative mRNA expression levels in comparison with Rpl13a are shown. Error bars represent SD. Significant difference from the control by Student’s t test (* p < 0.01). (B) After treatment as in A, LMW-PTP protein expression was analyzed by Western blotting. (C) MC3T3-E1 cells were stimulated by 100 ng/ml OPN for 1 h. Cells were further incubated in PBS with 50 mM BS3 for 20 min at room temperature. Immunoprecipitation was performed with anti-OPN antibody. Precipitates and lysate from MC3T3-E1 cells (positive control) were separated by SDS–PAGE, followed by detection using anti-CD44 antibody.

Article Snippet: Cyclo α V β 3 -integrin binding cyclic RGD peptide was from Anaspec (Fremont, CA).

Techniques: Expressing, Quantitative RT-PCR, Comparison, Control, Western Blot, Incubation, Immunoprecipitation, Positive Control, SDS Page